Genetic Engineering

by Smita Rastogi , Neelam Pathak (Authors)

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Genetic Engineering

Genetic Engineering is designed to serve as a textbook for the students of biotechnology, life sciences, genetics, microbiology, biochemistry, and other related areas. Replete with comprehensive details, it will also serve as a reference for the research students in this field. Divided into four parts, the book provides in-depth coverage of all major topics in this area. The first part on fundamentals of genetic engineering includes concepts, such as gene cloning, enzymes used in genetic engineering, synthesis of nucleic acids, and techniques like gel electrophoresis and polymerase chain reaction. The second part on vectors used in gene cloning covers the basic biology of plasmids, ë phages, other cloning vectors, and the strategies adopted for their application in gene cloning. In the third part, generation and screening of recombinants are described. This part includes detailed discussions on DNA ligation, DNA delivery methods, construction of genomic and cDNA libraries, and screening of transformants. The fourth part on applied genetic engineering covers DNA and protein sequencing, molecular markers, gene silencing, antisense RNA technology, and in vitro mutagenesis. Safety regulations related to genetically modified organisms (GMOs) are also discussed. Written in a lucid style, the book is highly interactive, which will help readers understand the contents through visualization of concepts and techniques, and their practice.

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